Hepatitis A Antibody IgG, Serum
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Turnaround Time:
Routine: 2 days
Test Schedule:
7 days a week
Interpretation:
Nonreactive = Hepatitis A IgG antibodies not detected
Reactive = Hepatitis A IgG antibodies detected
Specimen Requirements:
2 ml blood collected in serum separator tube (SST). Send 0.5 mL serum aliquot.
Minimum volume = 250 uL
Stability:
Room Temperature (21 - 30°C) – 16 hours
Refrigerated (2-8 C) - 8 days
Specimen may be stored on or off the clot, or separator gel
Frozen -20°C or colder – 30 days
Remove specimen from clot or separator gel prior to storage
Pediatric Specimen Requirements:
2 ml blood collected in serum separator tube (SST). Send 0.5 mL serum aliquot.
Minimum volume = 250 uL
Stability:
Room Temperature (21 - 30°C) – 16 hours
Refrigerated (2-8 C) - 8 days
Specimen may be stored on or off the clot, or separator gel
Frozen -20°C or colder – 30 days
Remove specimen from clot or separator gel prior to storage
Reference Range:
Nonreactive
Comments:
Use this test to determine the immune status of individuals to Hepatitis A virus (HAV) infection.
This test is not intended for use in screening blood, plasma or tissue donors.
RPR with Reflex to Titer for Treatment Response or Reinfections
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
RPR Qualitative
If reactive, reflex to:
RPR Quant (Titer)
Additional charges will apply
Turnaround Time:
2 Days
Test Schedule:
7 days a week
Specimen Requirements:
4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top).
Remove 2.0 mL serum from clot tube and send in an aliquot tube.
Minimum volume = 1 mL serum
Storage/Transport Temperature: Refrigerated
Stability:
Refrigerated (2-8 C) - 5 days
If delay in testing, store frozen at -20 C or colder.
Avoid repeated freeze/thaw cycles.
Remove specimen from clot or separator gel prior to storage.
Pediatric Specimen Requirements:
4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top).
Remove 2.0 mL serum from clot tube and send in an aliquot tube.
Minimum volume = 1 mL serum
Storage/Transport Temperature: Refrigerated
Stability:
Refrigerated (2-8 C) - 5 days
If delay in testing, store frozen at -20 C or colder.
Avoid repeated freeze/thaw cycles.
Remove specimen from clot or separator gel prior to storage.
Reference Range:
RPR Qual: Nonreactive
RPR Quant (Titer): Less than 1:1
Comments:
Preferred test for monitoring treatment response in established syphilis patients. No TP-PA will be reflexed.
Hepatitis B Surface AG W/Reflex Confirmation
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
Hepatitis B Surface Antigen If Reactive reflex to:
Hepatitis B Surface Antigen, Confirmation (LAB102548)
Additional charges apply.
Turnaround Time:
Routine: 2 days, additional time for reflex testing.
Test Schedule:
7 days a week.
Interpretation:
Nonreactive = Specimen is considered negative for HBsAg.
Pending Confirmation = Screening test is reactive and pending confirmation, as hepatitis B surface antigen screening test may yield a false positive result.
Specimen Requirements:
4 ml blood collected in serum separator tube (SST) or EDTA tube. Send 2 mL serum or plasma aliquot.
Minimum volume = 250 uL, 1.5 mL is needed to include reflex testing.
Stability:
Room Temperature (15 - 30°C) – 24 hours
Refrigerated (2-8 C) - 6 days
Specimen may be stored on or off the clot, or separator gel.
Frozen -10°C or colder – 3 freeze/thaw cycles
Remove specimen from clot or separator gel prior to storage.
Pediatric Specimen Requirements:
4 ml blood collected in serum separator tube (SST) or EDTA tube. Send 2 mL serum or plasma aliquot.
Minimum volume = 250 uL, 1.5 mL is needed to include reflex testing.
Stability:
Room Temperature (15 - 30°C) – 24 hours
Refrigerated (2-8 C) - 6 days
Specimen may be stored on or off the clot, or separator gel.
Frozen -10°C or colder – 3 freeze/thaw cycles
Remove specimen from clot or separator gel prior to storage.
Reference Range:
Nonreactive
Comments:
Used for the qualitative detection of hepatitis B surface antigen (HBsAg). May be used to screen for HBV infection in pregnant women to identify neonates who are at risk for acquiring hepatitis B during the perinatal period. May be used to provide presumptive evidence of infection with the hepatitis B virus (HBV).
This test is not intended for use in screening blood, plasma or tissue donors.
Hepatitis B Surface Antigen, Confirmation is sent to AHPL for testing.
Synonyms:
HBS
HBSAG
Hep B Surf AG
Hep B Surf AG Confirmation
Syphilis Antibody Screen W/ Reflex To RPR, Titer, and TP-PA Confirm
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
Treponema Pallidum Antibody. If reactive, reflex to RPR Qualitative.
• Reactive reflex goes to RPR Quant (Titer)
• Nonreactive reflex = T.PALLIDUM AB By TP-PA
Additional charges will apply.
Turnaround Time:
Routine: 2 days, additional time for reflex testing.
Test Schedule:
7 days a week
Interpretation:
| Result | Final Interpretation |
| Non - Reactive | Non-reactive for treponema antibodies |
| Reactive | Reactive for treponema antibodies |
Related Links:
Specimen Requirements:
4.0 mL blood in serum separator tube (SST). Send 2 mL serum aliquot.
Minimum volume is 1.5 mL serum.
Storage/Transport Temperature: Refrigerated. Remove specimen from clot or separator gel prior to storage.
Stabile refrigerated (2 to 8 C) for 5 days. Stable frozen -20 C or colder for 8 to 30 days.
Pediatric Specimen Requirements:
*Do not order for patients less than 18 months. See comments below.*
4.0 mL blood in serum separator tube (SST). Send 2 mL serum aliquot.
Minimum volume is 1.5 mL serum.
Storage/Transport Temperature: Refrigerated. Remove specimen from clot or separator gel prior to storage.
Stabile refrigerated (2 to 8 C) for 5 days. Stable frozen -20 C or colder for 8 to 30 days.
Reference Range:
Treponema pallidum Antibodies: Nonreactive
RPR: Nonreactive
RPR Titer: Less than 1:1
Comments:
Recommended test for syphilis screen for patients 18 months old or older (reverse algorithm).
For patients younger than 18 months, refer to RPR With Reflex to Titer and TP-PA Confirmation (LAB00282)
T.PALLIDUM AB By TP-PA is sent to ARUP for testing. Treponema pallidum Antibody by TP-PA (Opens in a new window).
Immunoglobulin Panel
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
IgA, Serum
IgG, Serum
IgM, Serum
Turnaround Time:
Routine: 4 days
Test Schedule:
Monday, Wednesday, and Friday.
Specimen Requirements:
2 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. Send 1 mL aliquot refrigerated.
Stability:
Plasma is stable for 7 days refrigerated at 2-8 C
Pediatric Specimen Requirements:
Minimum volume = 0.5mL serum
Reference Range:
IgG 768 - 1632 (mg/dL)
IgA 68 - 408 (mg/dL)
IgM 35 – 263 (mg/dL)
Synonyms:
IgA
IgG
IgM
HIV AB/AG Screening w/Reflex to Quant
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
HIV AB/AG Screen. If Screen is Reactive, reflex to:
HIV-1 RNA Quant (LAB00779)
HIV Ab Confirmation
Turnaround Time:
Routine: 2 days, additional time for reflex testing.
Test Schedule:
HIV AB/AG Screen: 7 days a week
HIV RNA Quant: Tuesday and Friday
HIV AB Confirm: 7 days a week
Specimen Requirements:
6 mL LAVENDER top (EDTA) vacutainer. Purple top only for reflex to viral load testing. Send 1 mL aliquot of plasma refrigerated, do not aliquot all plasma off the cells. Send plasma and cells.
Stability:
Whole blood collected in EDTA tubes may be stored and/or transported for up to 24 hours at room temp or refrigerated (2 to 25 C) prior to plasma separation.
After plasma separation, refrigerated (2 to 8 C) plasma is stable for up to 7 days for HIV AB/AG Screen and HIV AB Confirm, 6 days for HIV RNA QUANT.
Pediatric Specimen Requirements:
Do not order for pediatric patients less than 2 years of age, see Comment section below.
Minimum volume for testing is 1 mL plasma, do not aliquot all plasma off the cells. Send plasma and cells.
Reference Range:
Nonreactive
Comments:
Billable EAP and CPT Codes: Reflexes create additional charges.
Do not order for pediatric patients less than 2 years of age. NIH guidelines recommend virologic assays (i.e. RNA tests) that directly detect HIV for diagnosis of HIV infection in infants younger than 2 years.
This test is not intended for use in screening blood, plasma or tissue donors.
Synonyms:
AIDS Antibody Screen
Acquired Immunodeficiency Syndrome
HIV Antibody Screen
HIV - 1,2 AB/ HIV-1 P24 AG Screen
HIV P-24 Antigen
Human Immunodeficiency Virus
P-24 Antigen
Monoclonal Protein Study – SPEP and Immunotyping
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Turnaround Time:
Routine: 7 days
Test Schedule:
Monday through Friday.
Specimen Requirements:
4 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. A minimum of 2.0 mL serum is needed.
Synonyms:
IFE
Immunoelectrophoresis
Immunofixation
Monoclonal Protein Detection Quantitation and Characterization
MGUS study
Monoclonal Protein Study
Multiple Myeloma Study
Protein ELP
Protein Electrophoresis
Serum Protein Electrophoresis
SPEP
Monoclonal Protein Study Expanded Panel, Serum
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Turnaround Time:
Routine: 6 days
Test Schedule:
SPEP reflex to Immunotyping: Monday through Friday.
Immunoglobulin Panel: Monday, Wednesday, and Friday.
Specimen Requirements:
Labels for two specimens will print.
SPEP reflex to Immunotyping: 4 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. A minimum of 2.0 mL serum is needed.
Immunoglobulin Panel Serum: 2 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. A minimum of 1.0 mL serum is needed.
Tacrolimus
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Turnaround Time:
Routine: 1 day
Test Schedule:
Monday - Sunday
Units:
Critical Values:
20 ng/mL or greater.
Specimen Requirements:
3 mL blood in a LAVENDER top tube (EDTA); redraw if specimen clotted. Specimen is stable for up to 7 days refrigerated (2 to 8 C). If testing is delayed more than 7 days, store frozen at -10 C or less.
Pediatric Specimen Requirements:
0.5 mL blood in a LAVENDER top tube (EDTA); redraw if specimen clotted. Specimen is stable for up to 7 days refrigerated (2 to 8 C). If testing is delayed more than 7 days, store frozen at -10 C or less.
Reference Range:
Therapeutic range: 5 to 15 ng/mL
Comments:
Samples for analysis of Tacrolimus should be collected 30 minutes to 1 hour prior to the next dose so that the measured concentration of drug represents trough level. Some other factors influencing therapeutic range, dose administered, and result interpretation include time since transplantation, the organ transplanted, co-administration of other immunosuppressants and interaction with other drugs that may increase or decrease Tacrolimus concentrations.
Methodology: Chemiluminescent microparticle immunoassay (CMIA)
Synonyms:
FK506 (Tacrolimus), Whole Blood
Sirolimus, Whole Blood
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Turnaround Time:
Routine: 1 day
Test Schedule:
Monday through Friday.
Units:
Specimen Requirements:
3 mL blood in a LAVENDER top tube (EDTA); redraw if specimen clotted. Specimen is stable for up to 7 days refrigerated (2 to 8 C). If testing is delayed more than 7 days, store frozen at -10 C or less.
Pediatric Specimen Requirements:
0.5 mL blood in a LAVENDER top tube (EDTA); redraw if specimen clotted. Specimen is stable for up to 7 days refrigerated (2 to 8 C). If testing is delayed more than 7 days, store frozen at -10 C or less.
Reference Range:
4 to 12 ng/mL
Comments:
Test performed by immunoassay using Abbott Architect i1000.
Kidney transplant (in combination with Cyclosporine): 4 to 12 ng/mL
Toxic value: greater than 25 ng/mL
Interpretive Data: A range of 12 to 20 ng/mL has been suggested for liver transplant. Samples for analysis of Sirolimus should be collected 30 minutes to 1 hour prior to the next dose so that the measured concentration of drug represents trough levels. The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose and/or type of transplanted organ.
Synonyms:
Rapamune
Rapamycin
Sirolimus Quantitation