LIT XM Draw

Orderable EAP code:

LAB00840

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 5 Days
Urgent: 12 Hours

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

50 mL blood in YELLOW top (ACD) tubes. Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

20 mL blood in YELLOW top (ACD) tubes. Room temperature specimens are stable for up to 7 days.

Comments:

Store room temperature if overnight. Test must be arranged in advance by calling the lab at 503-494-2892. This draw provides the cells needed for a flow crossmatch.

Synonyms:

Crossmatch

Crossmatch, Flow

Orderable EAP code:

LAB00845

Billable EAP Codes:

80002432 x 2

CPT Codes:

86825 x 2

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

24 hour on call.

Units:

Pos/Neg

Specimen Requirements:

5 mL blood in a RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

4 mL blood in a 4 mL RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Comments:

Test must be arranged in advance by calling the lab at 494-2892. A flow cytometric assay for the detection of anti-donor antibodies. Test may be adjusted to detect IgG, IgM or polyvalent antibodies. Target cells can be T- or B-lymphocytes.

Synonyms:

Flow Crossmatch

Anti-A Titer

Orderable EAP code:

LAB00842

Billable EAP Codes:

80002429 x 1

CPT Codes:

86886 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

10 mL blood in a RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

2 mL blood in a 4 mL RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Comments:

This agglutination test determines the titer of the IgG component of anti-A1 in red blood cell group O and group B individuals.

HLA-B7

Orderable EAP code:

LAB100422

Billable EAP Codes:

80002871 x 1

CPT Codes:

81374 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

6 mL blood in a LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Pediatric Specimen Requirements:

2 mL blood in a 3 mL LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Comments:

A DNA test (polymerase chain reaction-sequence specific primers, sequence specific oligonucleotides, or Next Generation Sequencing) for the detection of the HLA-B7 antigen.

HLA-A2

Orderable EAP code:

LAB100421

Billable EAP Codes:

80002870 x 1

CPT Codes:

81374 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

6 mL blood in a LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Pediatric Specimen Requirements:

2 mL blood in a 3 mL LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Comments:

A DNA test (polymerase chain reaction-sequence specific primers, sequence specific oligonucleotides, or Next Generation Sequencing) for the detection of the HLA-A2 antigen.

HLA-DQA High Resolution

Orderable EAP code:

LAB100375

Billable EAP Codes:

80002814 x 1

CPT Codes:

81382 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

6 mL blood in a LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Pediatric Specimen Requirements:

2 mL blood in a 3 mL LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Comments:

Common and well-documented allele determination of HLA-DQA using DNA technology (polymerase chain reaction - sequence specific primers, sequence specific oligonucleotides, or Next Generation Sequencing). 

Synonyms:

LIT HLA-DQA High Resolution

HLA-B27

Orderable EAP code:

LAB00772

Billable EAP Codes:

80002430 x 1

CPT Codes:

81374 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Interpretation:

The presence of HLA-B27 is associated with ankylosing spondylitis and with acute anterior uveitis. Reference: Tiwari, Jawahar L.; Paul I. Terasaki. HLA and Disease Associations. Springer-Verlag, New York, Inc; 1985.

Specimen Requirements:

6 mL blood in a LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Pediatric Specimen Requirements:

2 mL blood in a 3 mL LAVENDER top (EDTA) tube. Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Comments:

A DNA test (polymerase chain reaction-sequence specific primers, sequence specific oligonucleotides, or Next Generation Sequencing) for the detection of the HLA-B27 antigen.

LIT HLA Class II Antibody ID

Orderable EAP code:

LAB00927

Billable EAP Codes:

80002456 x 1

CPT Codes:

86833 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Units:

Specificities if determined

Specimen Requirements:

5 mL blood in a RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

2 mL blood in a 4 mL RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Comments:

A flow cytometric assay is performed to determine specificity of IgG anti-class II HLA antibodies. 
*Typically ordered in conjunction with LIT HLA Class I Antibody ID test. Order combined test: LIT HLA Class I & II Antibody ID (LAB101444)

Synonyms:

FLOW HLA Class II AB Antigen ID High Def Qual, Class II ID
Donor Specific Antibody
DSA

LIT HLA Class I Antibody ID

Orderable EAP code:

LAB00925

Billable EAP Codes:

80002455 x 1

CPT Codes:

86832 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Units:

Specificities if determined

Specimen Requirements:

5 mL blood in a RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

2 mL blood in a 4 mL RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Comments:

A flow cytometric assay is performed to determine specificity of IgG anti-class I HLA antibodies. 
*Typically ordered in conjunction with LIT HLA Class II Antibody ID test. Order combined test: LIT HLA Class I & II Antibody ID (LAB101444)

Synonyms:

FLOW HLA I AB Antigen ID, Class I ID
Donor Specific Antibody
DSA

PRA Class I/II Antibody Screen

Orderable EAP code:

LAB01173

Billable EAP Codes:

80002457 x 1

CPT Codes:

86828 x 1

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 7 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Units:

% positive; specificities if determined

Specimen Requirements:

5 mL blood in a RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Pediatric Specimen Requirements:

2 mL blood in a 4 mL RED top tube. Refrigerated and Room temperature specimens are stable for up to 7 days.

Comments:

Refrigerate if overnight. A flow cytometric assay is performed to determine the presence, and possible specificity of IgG anti-class I and class II HLA antibodies. May be used to determine the probability of a patient being positive in the flow crossmatch against a random donor.

Synonyms:

FLOW HLA AB PRA SCR I/II