The Crest V3 is a spinning disk confocal on an inverted Nikon stand equipped with the following:

• Temperature and CO2 control for live sample imaging
• Widefield and confocal modes
• Spinning disk head with 50um pinhole, 250um spacing 
• Laser lines (please reach out for recommendations on channel combination): 
  
  • 405nm (DAPI)
  • 446nm (CFP)
  • 477nm (Cy2/GFP)
  • 518nm (YFP)
  • 545nm (Cy3/RFP)
  • 637nm (Cy5)
  • 748nm (Cy7)
• Objectives: 
  
  • 4x 0.2NA Air WD 20mm
  • 10x 0.45NA Air WD 4mm
  • 20x 0.80NA Air WD 0.8mm
  • 40x 0.95NA Air WD 0.25mm
  • 40x LWD 1.15NA Water WD 0.6mm
  • 60x 1.42NA Oil WD 0.15mm
  • 40x 1.3NA Oil WD 0.2mm (upon request) 
• Automated stage for large tiling and z-stack acquisition
• Large field of view sCMOS camera (Teledyne Kinetix)
• Stage inserts for accommodating:
  
  • Standard slides
  • Chambered slides
  • Round dishes
  • Multiwell plates

Please reach out to almc@ohsu.edu with inquiries.