The Crest V3 is a spinning disk confocal on an inverted Nikon stand equipped with the following:

• Temperature and CO2 control for live sample imaging
• Widefield and confocal modes
• Up to five fluorescent channels per experiment
  
  • Combination one: 
    
    • Blue: DAPI/AF405
    • Green: Cy2/AF488/GFP
    • Orange-red: Cy3/AF555/RFP
    • Far red: Cy5/AF647
    • Near infrared: Cy7/AF750
  • Also available:
    
    • Cyan: CFP
    • Yellow: YFP

Please reach out for staff recommendations on combining channels

• Objectives: 
  
  • 4x 0.2NA dry lens
  • 10x 0.45NA dry lens
  • 20x 0.80NA dry lens
  • 40x 0.95NA dry lens
  • 40x 1.15NA water lens
  • 40x 1.3NA multi-immersion lens
  • 60x 1.42NA oil lens
• Automated stage for large tiling and z-stack acquisitions 
• Stage inserts to accommodate:
  
  • Standard slides
  • 8 chambered slides
  • Round dishes
  • Multiwell plates of varying sizes

Please reach out to almc@ohsu.edu with inquiries.