HIT Antibody with Reflex to SRA Confirmation
Orderable EAP code:
Billable EAP Codes:
CPT Codes:
Lab Section:
Includes:
Preliminary positives are reflexed to Serotonin Release Assay (Heparin Dependent Platelet Antibody), Unfractionated Heparin at ARUP.
Link to ARUP Test Directory (Opens in a new window) for the SRA confirmation reflex test.
Turnaround Time:
Routine: 2 Hours
Urgent: 1 Hour
Test Schedule:
Batched daily 7 days per week. Please call 503-494-5764 if STAT.
Critical Values:
Preliminary Positive, Confirmation Pending
Specimen Requirements:
One 2.7 mL Lt Blue Na Citrate tube and one 5 mL Gold SST tube.
Pediatric Specimen Requirements:
One 1.3 mL Lt Blue Na Citrate tube and two pediatric red top tubes.
Reference Range:
HIT Ab Negative
Comments:
Method: Immunoturbidimetric detection of anti-PF4/Heparin (PF4/H) antibodies.
Interferences:
The HIT Ab assay is not affected by hemoglobin up to 500 mg/dL, bilirubin up to 19 mg/dL, triglycerides up to 375 mg/dL, rheumatoid factor up to 100 IU/mL and human anti-mouse antibodies (HAMA) up to 1 microgram/mL.
No dose-response correlation between HIT results and heparin concentrations was shown in manufacturer studies; UFH (n=11) range from 0.04-1.08 IU/mL and LMWH (n=115) range from 0-2.47 IU/mL).
Testing on 40 samples from patients diagnosed with Antiphospholipid Syndrome (APS) showed no HIT Ab positive results indicating that the assay is not affected by APS antibodies.
Synonyms:
Heparin Induced Thrombocytopenia Antibody