Complement and IgG antihuman globulin.

Routine: 2 hours
Urgent: 1 hour

Available 24 hours a day.

6.0 mL blood in a LAVENDER top tube. Specimen stability: up to 24 hours at room temperature.
See link for instructions for identification and labeling of Transfusion Service specimens. 
Sample Information (Opens in a new window)

1.0 mL blood in a LAVENDER top tube. (Additional sample may be required for elution testing). Specimen stability: up to 24 hours at room temperature.

Also known as DAT. Demonstrates the presence of immunoglobulins, complement, or protein adsorbed to red cells in vivo. Of value in the diagnosis and evaluation of hemolytic anemia, hemolytic disease of the newborn, suspected transfusion reactions, and in some diseases. Some medications can cause a positive test; rarely do such positive tests result in clinical hemolysis. Most common among the drugs reported to have caused positive direct antiglobulin tests are: Aldomet, penicillin, cephalosporins, INH, quinidine. Many hospitalized patients demonstrate weakly positive direct antiglobulin tests of no clinical significance.

Detects the presence of red blood cell-bound IgG and/or complement by agglutination using an antiglobulin (Coombs) reagent. Positive results can occur in patients with autoimmune hemolytic anemia, drug-induced immune hemolysis (e.g., alpha-methyldopa, procainamide, cepalosporins and penicillins, INH, quinidine, etc.), hemolytic transfusion reactions, or hemolytic disease of the newborn. Positive results can also occur due to non-specific IgG absorption (e.g., hypergamma-globulinemia), and occasionally in otherwise healthy individuals. Extended evaluation may include elution and absorption studies.

AHG Complement Direct Coombs
Anti-Human Globulin Test
Antiglobulin Test, Direct
Coombs, Direct
DAT
Direct Antiglobulin Test