Platelet Refractory Workup

Orderable EAP code:

LAB00220

Billable EAP Codes:

80002459 x 1 (HLA-A Low Res)
80002460 x 1 (HLA-B Low Res)
80002455 x 1 (LIT HLA Class I Antibody ID)

CPT Codes:

81373 x 2 (HLA-A Low Res, HLA-B Low Res)
86832 x 1 (LIT HLA Class I Antibody ID)

Lab Section:

Laboratory of Immunogenetics and Transplantation (LIT)

Turnaround Time:

Routine: 5 Days
Urgent: 2 Days

Test Schedule:

Monday through Friday from 7:30 am to 5:00 pm.

Specimen Requirements:

6 mL blood in a LAVENDER top EDTA tube AND 6 mL blood in a RED top tube (refrigerate if overnight, room temperature otherwise).
Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Pediatric Specimen Requirements:

2 mL blood in a 3mL LAVENDER top EDTA tube AND 1 mL blood in a 4 mL RED top tube (refrigerate if overnight, room temperature otherwise).
Refrigerated specimens are stable for up to 7 days. Room temperature samples are stable for up to 24 hours.

Comments:

Order as a follow-up when patient is refractory to platelet transfusion and nonimmune causes have been ruled out. HLA typing and antibody screen will be included, as appropriate. Results will be faxed to Transfusion Services and Red Cross Tissue Typing Department.
Determination of HLA-A and HLA-B histocompatibility antigens using DNA technology (polymerase chain reaction-sequence specific primers, sequence specific oligonucleotides, or Next Generation Sequencing).
A flow cytometric assay is performed to determine the specificity of IgG anti-class I antibodies.