Integrated Pathology Core

Retinal image with multiplex fluorescence staining: credit Jonathan Stoddard, Neuringer Laboratory

About us

The Integrated Pathology Core (IPC) provides investigators with state-of-the-art histology and advanced optical light microscopy imaging and analysis through extensive expertise, services, data analysis, and access to advanced equipment. Services provided include routine histology, cryosectioning, immunohistochemistry, next-generation RNAScope/DNAScope in situ hybridization, advanced optical light (bright field and multi-spectral confocal) microscopy, digital whole-slide imaging, and quantitative image analysis. Core personnel are available at every stage of histology and microscopy-based experiments, from planning to training, troubleshooting, and quality control.

The Core has an extensive resource of tissue-based analyses and techniques including multiplexed immunohistochemical and immunofluorescence staining and RNA/DNAScope in situ hybridization. In addition to routine and automated histology equipment, advanced histology techniques are supported by a Biocare IntelliPATH automated stainer for immunohistochemistry. Imaging and microscopy capabilities include a Leica Stellaris 8 confocal, Leica Aperiao AT2 bright field slide scanner, Olympus VS120 fluorescence (up to 5 channels) and Bright field slide scanner with multiple slide inserts, and a Keyence BZ-X810 Fluorescence Microscope.  Image analysis is supported by multiple platforms including the HALO Image Analysis suite (Indica Lab, Corrales, NM), an advanced quantitative image analysis package that supports unique tissue-specific and disease-specific applications.  

Contact us

Director, Integrated Pathology Core
Oleg Varlamov, MD, PhD
varlamov@ohsu.edu
503-346-5377

Histology services
Rachel Dannay

dannay@ohsu.edu
503-346-5077

Advanced Microscopy services
Sathya Srinivansan, PhD
srinivsa@ohsu.edu
503-346-5072

Shipping address
Integrated Pathology Core
Oregon National Primate Research Center
Campus Mail Code L-584
505 NW 185th Avenue
Beaverton, OR 97006

Integrated Pathology Core Services

  • Pre-project planning
  • Expert consultation
  • Method documentation
  • Publication preparation

  • Routine and customized tissue processing
  • Embedding, sectioning (including large format blocks)
  • Routine hematoxylin and eosin staining
  • Frozen sectioning
  • Custom sectioning: serial, step, large format
  • Special histochemical stains
  • Cassette and slide printing

IPC_Histology_automated stainer
  • Bench top and automated immunohistochemistry
  • Antibody optimization
  • Multiplex immunohistochemistry
  • RNAscope and DNAscope In Situ Hybridization
  • Extensive portfolio of antibodies and In situ Hybridization probes validated for use in macaques

  • Tissue processors
  • Leica cassette labeler
  • Leica slide printer, automated cover-slipper, ans auto-stainer
  • Semi-automated and manual rotary microtomes
  • Biocare IntelliPATH automated immunohistochemistry platform

IPC_Imaging_Aperio whole slide scanner
  • Fluorescence imaging: confocal, wide-field, and deconvolution microscopy
  • Special fluorescence techniques: FURA-2, FRET, FRAP, and live-cell time-lapse imaging
  • Large-area image montages: color bright field or fluorescence, including 3D confocal
  • Stereology image analysis
  • Image processing, 3D rendering, movies
  • Quantitative image analysis and stereology

  • Leica Aperio AT2 400 slide scanner (bright field)
  • Olympus VS120 bright field and fluorescent (up to 5 channels) slide scanner
  • Leica Stellaris 8 confocal microscope
  • MBF Bioscience Stereology System (offline analysis)

  • HALO Image Analysis: HALO, HALOLink
  • MBF Bioscience Stereology System with StereoInvestigators and NeuroLucida
  • Olympus CellSens
  • QuPath
  • FIJI/ ImageJ