Francis I. Valiyaveetil, Ph.D.
- Professor of Chemical Physiology and Biochemistry, School of Medicine
- Neuroscience Graduate Program, School of Medicine
- Graduate Program in Biomedical Sciences, School of Medicine
Biography
Probing Functional Mechanisms in Channels and Transporters
We are investigating functional mechanisms that govern molecular recognition and conformational dynamics in ion channels and transporters. In our investigations, we use a multidisciplinary approach that combines unnatural mutagenesis with structural studies using x-ray crystallography. We also carry out functional measurements using electrophysiology and investigate protein dynamics using EPR and fluorescence spectroscopy.
Education and training
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Degrees
- B.S., 1990, Chemistry, University of Mumbai, India
- M.S., 1992, Biotechnology, IIT-Mumbai, India
- Ph.D., 1999, Biomolecular Chemistry, University of Wisconsin-Madison
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Fellowship
- 2005 Postdoctoral (Molecular Neurobiology and Peptide Chemistry), Rockefeller University
Areas of interest
- Unnatural amino acid mutagenesis of membrane proteins
- Ion conduction and voltage gating in K+ channels
- Transport mechanism in glutamate transporters
- Folding of membrane proteins
Additional information
Honors and awards
- 2007 - Pew Scholar Award
- 1992 - Cremer Research Fellowship
Publications
Publications
A facile approach for incorporating tyrosine esters to probe ion-binding sites and backbone hydrogen bonds
Journal of Biological ChemistryWater inside the Selectivity Filter of a K+ Ion Channel
Journal of the American Chemical SocietyProbing Ion Configurations in the KcsA Selectivity Filter with Single-Isotope Labels and 2D IR Spectroscopy
Journal of the American Chemical SocietyDistinct roles of the Na+ binding sites in the allosteric coupling mechanism of the glutamate transporter homolog, GltPh
Proceedings of the National Academy of Sciences of the United States of AmericaStructural basis for C-type inactivation in a Shaker family voltage-gated K+ channel
Science AdvancesStructures of Gating Intermediates in a K+ channell
Journal of molecular biologyA Shared Mechanism for the Folding of Voltage-Gated K + Channels
BiochemistryInvestigation of the allosteric coupling mechanism in a glutamate transporter homolog via unnatural amino acid mutagenesis
Proceedings of the National Academy of Sciences of the United States of AmericaA facile approach for the in vitro assembly of multimeric membrane transport proteins
eLifeMain-chain mutagenesis reveals intrahelical coupling in an ion channel voltage-sensor
Nature communicationsProbing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy
Journal of the American Chemical SocietyCombining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression
BiochemistryIndividual Ion Binding Sites in the K+ Channel Play Distinct Roles in C-type Inactivation and in Recovery from Inactivation
StructureInstantaneous ion configurations in the K+ion channel selectivity filter revealed by 2D IR spectroscopy
ScienceEngineering the glutamate transporter homologue GltPh using protein semisynthesis
BiochemistryIon-binding properties of a K+ channel selectivity filter in different conformations
Proceedings of the National Academy of Sciences of the United States of AmericaSemisynthetic K+ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state
Proceedings of the National Academy of Sciences of the United States of AmericaUsing protein backbone mutagenesis to dissect the link between ion occupancy and C-Type inactivation in K+ channels
Proceedings of the National Academy of Sciences of the United States of AmericaIn vitro folding of K vAP, a voltage-gated K + channel
BiochemistrySemisynthesis of NaK, a Na+ and K+ conducting ion channel
BiochemistryStudies of ion channels using expressed protein ligation
Current Opinion in Chemical BiologyModular strategy for the semisynthesis of a K+ channel
ACS chemical biologyIon selectivity in a semisynthetic K+ channel locked in the conductive conformation
ScienceStructural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel
Journal of the American Chemical Society