Stefanie Kaech Petrie, Ph.D.

  • Associate Professor of Neurology, School of Medicine
  • Director, Advanced Light Microscopy Core
  • Neuroscience Graduate Program, School of Medicine
  • Jungers Center for Neurosciences Research

Biography

Research Interests

The first time I watched a time-lapse recording of a living cell, I became fascinated by the power of digital microscopy. The discovery of GFP and its application to visualize dynamic events inside cells has revolutionized modern cell biology. I first used this enabling technology to study how structural proteins give a neuron its shape. Arriving in Portland, I began investigating how motor proteins traffick membrane proteins along neuronal axons and dendrites. I also began to share my knowledge about the uses of light microscopy in basic research by teaching OHSU researchers how to operate shared microscope equipment. In my current role as the Director of the Advanced Light Microscopy Core, the university-wide shared resource for light microscopy, I get to discuss imaging projects with students, postdocs, and faculty alike. We teach them the proper use of the high-end equipment in the Core and advise on sample preparation and data analysis. The most rewarding aspect of my work is that I get to share their excitement when the images captured provide visual and quantitative answers to their scientific pursuits.

Biography

Dr. Kaech Petrie obtained her Ph.D. in Cell Biology in 1991 at the University of Basel, Switzerland. After a short post-doctoral experience at NINDS-NIH in Bethesda, Maryland, she returned to the Friedrich Miescher Institute in Basel, Switzerland, where she specialized in the visualization of dynamic processes in living cells using GFP, a technology just emerging at that time. She joined OHSU in 2000 and transitioned to the Director of the Advanced Light Microscopy Core at The Jungers Center in 2009.

Education and training

  • Degrees

    • Ph.D., 1991, University of Basel

Areas of interest

  • Neuronal cell biology

Publications

Publications

  • SMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins

    JACS Au
    1. Julia Warren
    2. Jian Wang
    3. Francis Dhoro
    4. Bo Chao
    5. Ashok Reddy
    6. Stefanie Kaech Petrie
    7. Larry L. David
    8. Xiangshu Xiao
    9. Bingbing X. Li

  • CG-SLENP

    JACS Au
    1. Jian Wang
    2. Bo Chao
    3. Jake Piesner
    4. Felice Kelly
    5. Stefanie Kaech Petrie
    6. Xiangshu Xiao
    7. Bingbing X. Li

  • Large-scale recording of neuronal activity in freely-moving mice at cellular resolution

    Nature communications
    1. Aniruddha Das
    2. Sarah Holden
    3. Julie Borovicka
    4. Jacob Icardi
    5. Abigail O’Niel
    6. Ariel Chaklai
    7. Davina Patel
    8. Rushik Patel
    9. Stefanie Kaech Petrie
    10. Jacob Raber
    11. Hod Dana

  • A clickable photoaffinity probe of betulinic acid identifies tropomyosin as a target

    Acta Pharmaceutica Sinica B
    1. Pedro Martín-Acosta
    2. Qianli Meng
    3. John Klimek
    4. Ashok P. Reddy
    5. Larry David
    6. Stefanie Kaech Petrie
    7. Bingbing X. Li
    8. Xiangshu Xiao

  • Creating a career path for shared research resources personnel

    Journal of Biomolecular Techniques
    1. Luellen Fletcher
    2. Christina A. Harrington
    3. Aaron Nilsen
    4. Stefanie Kaech Petrie
    5. Andrew I. Chitty

  • Resistance Profile and Structural Modeling of Next-Generation ROS1 Tyrosine Kinase Inhibitors

    Molecular cancer therapeutics
    1. Clare Keddy
    2. Pushkar Shinde
    3. Kristen Jones
    4. Stefanie Kaech
    5. Romel Somwar
    6. Ujwal Shinde
    7. Monika A. Davare

  • Deletion of Stim1 in Hypothalamic Arcuate Nucleus Kiss1 Neurons Potentiates Synchronous GCaMP Activity and Protects against Diet-Induced Obesity

    Journal of Neuroscience
    1. Jian Qiu
    2. Todd L. Stincic
    3. Martha A. Bosch
    4. Ashley M. Connors
    5. Stefanie Kaech Petrie
    6. Oline K. Rønnekleiv
    7. Martin J. Kelly

  • High-fidelity, efficient, and reversible labeling of endogenous proteins using crispr-based designer exon insertion

    eLife
    1. Haining Zhong
    2. Cesar C. Ceballos
    3. Crystian I. Massengill
    4. Michael A. Muniak
    5. Lei Ma
    6. Maozhen Qin
    7. Stefanie Kaech Petrie
    8. Tianyi Mao

  • Selective microtubule-based transport of dendritic membrane proteins arises in concert with axon specification

    Journal of Neuroscience
    1. Jennifer D. Petersen
    2. Stefanie Kaech
    3. Gary Banker

  • Super-resolution imaging of neuronal dense-core vesicles

    Journal of Visualized Experiments
    1. Bethe A. Scalettar
    2. Daniel Shaver
    3. Stefanie Kaech
    4. Janis E. Lochner

  • FANCL ubiquitinates β-catenin and enhances its nuclear function

    Blood
    1. Kim Hien T. Dao
    2. Michael D. Rotelli
    3. Curtis L. Petersen
    4. Stefanie Kaech
    5. Whitney D. Nelson
    6. Jane E. Yates
    7. Amy E. Hanlon Newell
    8. Susan B. Olson
    9. Brian J. Druker
    10. Grover C. Bagby

  • General considerations for live imaging of developing hippocampal neurons in culture

    Cold Spring Harbor Protocols
    1. Stefanie Kaech
    2. Chun Fang Huang
    3. Gary Banker

  • Long-term time-lapse imaging of developing hippocampal neurons in culture

    Cold Spring Harbor Protocols
    1. Stefanie Kaech
    2. Chun Fang Huang
    3. Gary Banker

  • Regulation of neuronal mRNA translation by CaM-Kinase I phosphorylation of eIF4GII

    Journal of Neuroscience
    1. Taasin Srivastava
    2. Dale A. Fortin
    3. Sean Nygaard
    4. Stefanie Kaech
    5. Nahum Sonenberg
    6. Arthur M. Edelman
    7. Thomas R. Soderling

  • Short-term high-resolution imaging of developing hippocampal neurons in culture

    Cold Spring Harbor Protocols
    1. Stefanie Kaech
    2. Chun Fang Huang
    3. Gary Banker

  • Expression of kinesin superfamily genes in cultured hippocampal neurons

    Cytoskeleton
    1. M. A. Silverman
    2. S. Kaech
    3. E. M. Ramser
    4. X. Lu
    5. M. R. Lasarev
    6. S. Nagalla
    7. G. Banker

  • Posttranslational modifications of tubulin and the polarized transport of kinesin-1 in neurons

    Molecular biology of the cell
    1. Jennetta W. Hammond
    2. Chun Fang Huang
    3. Stefanie Kaech
    4. Catherine Jacobson
    5. Gary Banker
    6. Kristen J. Verhey

  • The interaction of Epac1 and ran promotes Rap1 activation at the nuclear envelope

    Molecular and cellular biology
    1. Chang Liu
    2. Maho Takahashi
    3. Yanping Li
    4. Tara J. Dillon
    5. Stefanie Kaech
    6. Philip J.S. Stork

  • Automated methods for profiling the axonal transport of secretory bdnf granules in live cultured neurons from time-lapse microscopy data

    Microscopy and Microanalysis
    1. Amit Mukherjee
    2. Andrew Cohen
    3. Badrinath Roysam
    4. Brian Jenkins
    5. Cheng Fang
    6. Stefanie Kaech Petrie
    7. Gary Banker

  • Transient receptor potential canonical 5 channels activate Ca 2+/calmodulin kinase Iγ to promote axon formation in hippocampal neurons

    Journal of Neuroscience
    1. Monika A. Davare
    2. Dale A. Fortin
    3. Takeo Saneyoshi
    4. Sean Nygaard
    5. Stefanie Kaech
    6. Gary Banker
    7. Thomas R. Soderling
    8. Gary A. Wayman

  • A WAVE-1 and WRP signaling complex regulates spine density, synaptic plasticity, and memory

    Journal of Neuroscience
    1. Scott H. Soderling
    2. Eric S. Guire
    3. Stefanie Kaech
    4. Jon White
    5. Fang Zhang
    6. Kevin Schutz
    7. Lorene K. Langeberg
    8. Gary Banker
    9. Jacob Raber
    10. John D. Scott

  • Putting on the RITz.

    Science's STKE : signal transduction knowledge environment
    1. Stefanie Kaech
    2. Gary Banker
    3. Phillip Stork

  • Culturing hippocampal neurons

    Nature protocols
    1. Stefanie Kaech
    2. Gary Banker

  • Influx of extracellular calcium regulates actin-dependent morphological plasticity in dendritic spines

    Neuropharmacology
    1. Ina Brünig
    2. Stefanie Kaech
    3. Heike Brinkhaus
    4. Thomas G. Oertner
    5. Andrew Matus

  • Regulation of Axonal Extension and Growth Cone Motility by Calmodulin-Dependent Protein Kinase I

    Journal of Neuroscience
    1. Gary A. Wayman
    2. Stefanie Kaech
    3. Wilmon F. Grant
    4. Monika Davare
    5. Soren Impey
    6. Hiroshi Tokumitsu
    7. Naohito Nozaki
    8. Gary Banker
    9. Thomas R. Soderling

  • Dynamic properties of APC-decorated microtubules in living cells

    Cell Motility and the Cytoskeleton
    1. Rejith Dayanandan
    2. Rachel Butler
    3. Phillip R. Gordon-Weeks
    4. Andrew Matus
    5. Stefanie Kaech
    6. Simon Lovestone
    7. Brian H. Anderton
    8. Jean Marc Gallo

  • Two distinct mechanisms target membrane proteins to the axonal surface

    Neuron
    1. Bernard Sampo
    2. Stefanie Kaech
    3. Stefan Kunz
    4. Gary Banker

  • Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane

    Science
    1. S. M. Maira
    2. I. Galetic
    3. D. P. Brazil
    4. S. Kaech
    5. E. Ingley
    6. M. Thelen
    7. B. A. Hemmings

  • Cytoskeletal microdifferentiation

    Proceedings of the National Academy of Sciences of the United States of America
    1. Stefanie Kaech
    2. Hema Parmar
    3. Martijn Roelandse
    4. Caroline Bornmann
    5. Andrew Matus