Anthony P. Barnes, Ph.D.

  • Assistant Professor of Medicine, Division of Cardiovascular Medicine, School of Medicine
  • Neuroscience Graduate Program, School of Medicine
  • Program in Molecular and Cellular Biosciences, School of Medicine

Biography

The Barnes Lab focuses on two fundamental cellular phenomena. One set of projects in lab is directed at understanding the nature and purpose of endothelial heterogeneity as well as how the differences in an otherwise homogenous cell type contribute to cardiac disease. Our second focus centers on the genetic and cellular mechanisms that control the development of the cerebral cortex prior to and following birth to better understand the fundamental basis of neurodevelopmental disease.

Toward our vascular biology goals, we are applying a number of cellular and molecular methodologies to understand how (cell specification mechanisms) and why (physiologic significance) the cells that form the inner lining of all blood vessels display distinct molecular identities. These differences between endothelia are often a consequence of distinct functional requirements of the organ which they serve. Our goal is to understand the genetic basis for these unique populations and how these differences may shape the response of the vasculature to disease processes and contribute to pathologic outcome in the circulatory system.

For our developmental neuroscience mission, we concentrate on how signal transduction shapes the brain patterning and neuronal maturation. The cells of the developing nervous system are bombarded by a myriad of external signals during their migration and differentiation. Deciphering how these cells interpret and respond to these signals provides insight into the mechanisms shaping brain development and responses following injury. The Barnes laboratory identifies and characterizes genes that are required for the proper formation and connectivity of the developing cerebral cortex. A major focus of the lab is directed toward mapping the pathways that link extra-cellular stimuli to alterations in gene expression and cytoskeletal rearrangement during neuronal differentiation. Neuronal polarity is a fundamental aspect of differentiation and function. In the last decade, neuroscientists have begun to discover the signaling cascades crucial for the ultimate establishment of the structural and functional distinctions of dendrites and axons. The laboratory combines an array of techniques from conditional gene deletion to cell biology and proteomics to probe the role of various signals in neuronal polarization.

Areas of interest

  • Brain Development, Radial Glia, Neuronal Polarity, Endothelial heterogeneity, Single Cell Transcriptome/Proteome Analysis

Publications

Publications

  • Longitudinal MRI of the developing ferret brain reveals regional variations in timing and rate of growth

    Cerebral Cortex
    1. Kara E. Garcia
    2. Xiaojie Wang
    3. Sarah E. Santiago
    4. Stuti Bakshi
    5. Anthony Barnes
    6. Christopher D. Kroenke
  • Development of a novel Guinea Pig model producing transgenerational endothelial transcriptional changes driven by maternal food restriction and a second metabolic insult of high fat diet

    Frontiers in Physiology
    1. Hillary H. Le
    2. Matthew W. Hagen
    3. Samantha Louey
    4. Hagai Tavori
    5. Kent L. Thornburg
    6. George D. Giraud
    7. Monica T. Hinds
    8. Anthony P. Barnes
  • GPR39 Knockout Worsens Microcirculatory Response to Experimental Stroke in a Sex-Dependent Manner

    Translational Stroke Research
    1. Yifan Xu
    2. Wenri H. Zhang
    3. Elyse M. Allen
    4. Lev M. Fedorov
    5. Anthony P. Barnes
    6. Zu Yuan Qian
    7. Thierno Madjou Bah
    8. Yuandong Li
    9. Ruikang K. Wang
    10. Robert E. Shangraw
    11. Nabil J. Alkayed
  • Control of coronary vascular resistance by eicosanoids via a novel GPCR

    American Journal of Physiology - Cell Physiology
    1. Sanjiv Kaul
    2. Anthony P. Barnes
    3. Nabil J. Alkayed
    4. Zhiping Cao
    5. Zu Yuan Qian
    6. Shanthi Nagarajan
    7. Xuehong Liu
    8. Jonathan W. Nelson
    9. Fuchun Xie
    10. Bingbing Li
    11. Wei Fan
    12. Lijuan Liu
    13. Marjorie R. Grafe
    14. Xiangshu Xiao
    15. Catherine M. Davis
  • Horizontal mtDNA transfer between cells is common during mouse development

    iScience
    1. Nuria Marti Gutierrez
    2. Aleksei Mikhalchenko
    3. Hong Ma
    4. Amy Koski
    5. Ying Li
    6. Crystal Van Dyken
    7. Rebecca Tippner-Hedges
    8. David Yoon
    9. Dan Liang
    10. Tomonari Hayama
    11. David Battaglia
    12. Eunju Kang
    13. Yeonmi Lee
    14. Anthony Paul Barnes
    15. Paula Amato
    16. Shoukhrat Mitalipov
  • Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart

    Scientific Reports
    1. Zhiping Cao
    2. Jessica Minnier
    3. Lijuan Liu
    4. Kristin L.Lyon Scott
    5. Ashok P. Reddy
    6. Phillip A. Wilmarth
    7. Larry L. David
    8. Anthony P. Barnes
    9. Marjorie R. Grafe
    10. Sanjiv Kaul
    11. Nabil J. Alkayed
    12. Catherine M. Davis
  • Age-dependent transcriptional alterations in cardiac endothelial cells

    Physiological genomics
    1. Uchenna Emechebe
    2. Jonathan W. Nelson
    3. Nabil J. Alkayed
    4. Sanjiv Kaul
    5. Andrew C. Adey
    6. Anthony P. Barnes
  • Mapping the Molecular Architecture Required for Lipid-Binding Pockets Using a Subset of Established and Orphan G-Protein Coupled Receptors

    Journal of Chemical Information and Modeling
    1. Shanthi Nagarajan
    2. Zu Yuan Qian
    3. Parthiban Marimuthu
    4. Nabil J. Alkayed
    5. Sanjiv Kaul
    6. Anthony P. Barnes
  • (Phospho)Proteomic dataset of ischemia- and ultrasound- stimulated mouse cardiac endothelial cells in vitro

    Data in Brief
    1. Uchenna Emechebe
    2. David Giraud
    3. Azzdine Y. Ammi
    4. Kristin L. Scott
    5. Jon M. Jacobs
    6. Jason E. McDermott
    7. Igor V. Dykan
    8. Nabil J. Alkayed
    9. Anthony P. Barnes
    10. Sanjiv Kaul
    11. Catherine M. Davis
  • Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound

    Biochimica et Biophysica Acta - Proteins and Proteomics
    1. Uchenna Emechebe
    2. David Giraud
    3. Azzdine Y. Ammi
    4. Kristin L. Scott
    5. Jon M. Jacobs
    6. Jason E. McDermott
    7. Igor V. Dykan
    8. Nabil J. Alkayed
    9. Anthony P. Barnes
    10. Sanjiv Kaul
    11. Catherine M. Davis
  • Effect of thermostable mutations on the neurotensin receptor 1 (NTSR1) activation state

    Journal of Biomolecular Structure and Dynamics
    1. Shanthi Nagarajan
    2. Nabil J. Alkayed
    3. Sanjiv Kaul
    4. Anthony P. Barnes
  • Endothelial transcriptomics reveals activation of fibrosis-related pathways in hypertension

    Physiological genomics
    1. Jonathan W. Nelson
    2. Mohammed Z. Ferdaus
    3. James A. McCormick
    4. Jessica Minnier
    5. Sanjiv Kaul
    6. David H. Ellison
    7. Anthony P. Barnes
  • Specific gene expression profiles are associated with a pathologic complete response to neoadjuvant therapy in esophageal adenocarcinoma

    American journal of surgery
    1. Patrick J. McLaren
    2. Anthony P. Barnes
    3. Willy Z. Terrell
    4. Gina M. Vaccaro
    5. Jack Wiedrick
    6. John G. Hunter
    7. James P. Dolan
  • The START App

    Bioinformatics
    1. Jonathan W. Nelson
    2. Jiri Sklenar
    3. Anthony P. Barnes
    4. Jessica Minnier
  • Disrupting dimerization translocates soluble epoxide hydrolase to peroxisomes

    PloS one
    1. Jonathan W. Nelson
    2. Anjali J. Das
    3. Anthony P. Barnes
    4. Nabil J. Alkayed
  • Isl1 Directly Controls a Cholinergic Neuronal Identity in the Developing Forebrain and Spinal Cord by Forming Cell Type-Specific Complexes

    PLoS genetics
    1. Hyong Ho Cho
    2. Francesca Cargnin
    3. Yujin Kim
    4. Bora Lee
    5. Ryuk Jun Kwon
    6. Heejin Nam
    7. Rongkun Shen
    8. Anthony P. Barnes
    9. Jae W. Lee
    10. Seunghee Lee
    11. Soo Kyung Lee
  • STRAD pseudokinases regulate axogenesis and LKB1 stability

    Neural Development
    1. Biliana O. Veleva-Rotse
    2. James L. Smart
    3. Annette F. Baas
    4. Benjamin Edmonds
    5. Zi ming Zhao
    6. Allyson Brown
    7. Lillian R. Klug
    8. Kelly Hansen
    9. Gabrielle Reilly
    10. Alexandria P. Gardner
    11. Krishnaveni Subbiah
    12. Eric A. Gaucher
    13. Hans Clevers
    14. Anthony P. Barnes
  • Cancer- and endotoxin-induced cachexia require intact glucocorticoid signaling in skeletal muscle

    FASEB Journal
    1. Theodore P. Braun
    2. Aaron J. Grossberg
    3. Stephanie M. Krasnow
    4. Peter R. Levasseur
    5. Marek Szumowski
    6. Xin Xia Zhu
    7. Julia E. Maxson
    8. J. Gabriel Knoll
    9. Anthony P. Barnes
    10. Daniel L. Marks
  • Expression of myeloid differentiation factor 88 in neurons is not requisite for the induction of sickness behavior by interleukin-1β

    Journal of Neuroinflammation
    1. Theodore P. Braun
    2. Aaron J. Grossberg
    3. Biliana O. Veleva-Rotse
    4. Julia E. Maxson
    5. Marek Szumowski
    6. Anthony P. Barnes
    7. Daniel L. Marks
  • An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors

    Molecular Neurodegeneration
    1. Justin M. Dean
    2. Art Riddle
    3. Jennifer Maire
    4. Kelly D. Hansen
    5. Marnie Preston
    6. Anthony P. Barnes
    7. Larry S. Sherman
    8. Stephen A. Back
  • TGF-β Signaling Specifies Axons during Brain Development

    Cell
    1. Jason J. Yi
    2. Anthony P. Barnes
    3. Randal Hand
    4. Franck Polleux
    5. Michael D. Ehlers
  • LKB1 and SAD Kinases Define a Pathway Required for the Polarization of Cortical Neurons

    Cell
    1. Anthony P. Barnes
    2. Brendan N. Lilley
    3. Y. Albert Pan
    4. Lisa J. Plummer
    5. Ashton W. Powell
    6. Alexander N. Raines
    7. Joshua R. Sanes
    8. Franck Polleux
  • Phosphodiesterase 4D forms a cAMP diffusion barrier at the apical membrane of the airway epithelium

    Journal of Biological Chemistry
    1. Anthony P. Barnes
    2. Gabriel Livera
    3. Pingbo Huang
    4. Chuanwen Sun
    5. Wanda K. O'Neal
    6. Marco Conti
    7. M. Jackson Stutts
    8. Sharon L. Milgram