pH-Induced Binding of the Axial Ligand in an Engineered CuA Site Favors the πu State.
Morgada MN, Emiliani F, Chacón KN, Álvarez-Paggi D, Murgida DH, Blackburn NJ, Abriata LA, Vila AJ. Inorg Chem. 2019 Dec 2;58(23):15687-15691. doi: 10.1021/acs.inorgchem.9b01868. Epub 2019 Nov 11. PubMed PMID: 31710470.
Catalytic M Center of Copper Monooxygenases Probed by Rational Design. Effects of Selenomethionine and Histidine Substitution on Structure and Reactivity.
Alwan KB, Welch EF, Blackburn NJ. Biochemistry. 2019 Nov 5;58(44):4436-4446. doi: 10.1021/acs.biochem.9b00823. Epub 2019 Oct 28. PMID: 31626532
The binuclear cluster of [FeFe] hydrogenase is formed with sulfur donated by cysteine of an [Fe(Cys)(CO)2(CN)] organometallic precursor.
Rao G, Pattenaude SA, Alwan K, Blackburn NJ, Britt RD, Rauchfuss TB. Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20850-20855. doi: 10.1073/pnas.1913324116. Epub 2019 Sep 30. PubMed PMID: 31570604; PubMed Central PMCID: PMC6800375.
Incorporation of Ni2+, Co2+, and Selenocysteine into the Auxiliary Fe-S Cluster of the Radical SAM Enzyme HydG.
Rao G, Alwan KB, Blackburn NJ, Britt RD. Inorg Chem. 2019 Oct 7;58(19):12601-12608. doi: 10.1021/acs.inorgchem.9b01293. Epub 2019 Sep 20. PubMed PMID: 31539235; PubMed Central PMCID: PMC6984664.
Rational Design of a Histidine-Methionine Site Modeling the M-Center of Copper Monooxygenases in a Small Metallochaperone Scaffold.
Alwan KB, Welch EF, Arias RJ, Gambill BF, Blackburn NJ. Biochemistry. 2019 Jul 16;58(28):3097-3108. doi: 10.1021/acs.biochem.9b00312. Epub 2019 Jun 27. PMID: 31243953
Trapping intermediates in metal transfer reactions of the CusCBAF export pump of Escherichia coli.
Chacón KN, Perkins J, Mathe Z, Alwan K, Ho EN, Ucisik MN, Merz KM, Blackburn NJ. Commun Biol. 2018 Nov 14;1:192. doi: 10.1038/s42003-018-0181-9. eCollection 2018. PMID: 30456313
Substrate-Induced Carbon Monoxide Reactivity Suggests Multiple Enzyme Conformations at the Catalytic Copper M-Center of Peptidylglycine Monooxygenase.
Kline CD, Blackburn NJ. Biochemistry. 2016 Dec 6;55(48):6652-6661. Epub 2016 Nov 22. PMID: 27933800
pH-regulated metal-ligand switching in the HM loop of ATP7A: a new paradigm for metal transfer chemistry.
Kline CD, Gambill BF, Mayfield M, Lutsenko S, Blackburn NJ. Metallomics. 2016 Aug 1;8(8):729-33. doi: 10.1039/c6mt00062b. PMID: 27242196
Stopped-Flow Studies of the Reduction of the Copper Centers Suggest a Bifurcated Electron Transfer Pathway in Peptidylglycine Monooxygenase.
Chauhan S, Hosseinzadeh P, Lu Y, Blackburn NJ. Biochemistry. 2016 Apr 5;55(13):2008-21. doi: 10.1021/acs.biochem.6b00061. Epub 2016 Mar 23. PMID: 26982589
Kβ Valence to Core X-ray Emission Studies of Cu(I) Binding Proteins with Mixed Methionine - Histidine Coordination. Relevance to the Reactivity of the M- and H-sites of Peptidylglycine Monooxygenase.
Martin-Diaconescu V, Chacón KN, Delgado-Jaime MU, Sokaras D, Weng TC, DeBeer S, Blackburn NJ. Inorg Chem. 2016 Apr 4;55(7):3431-9. doi: 10.1021/acs.inorgchem.5b02842. Epub 2016 Mar 11. PMID: 26965786